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A. Adawy, W. Corbeek, E. de Ronde, W.J.P. van Enckevort, W.J. de Grip, E. Vlieg,

A practical kit for micro-scale application of the ceiling crystallisation method,

Cryst. Eng. Comm. 17 (2015) 2602-2605

Abstract:

We designed a kit to facilitate the optimisation of protein crystal growth by means of the ceiling crystallisation method. This ceiling kit allows for diffusion-limited crystal growth with a total volume down to 10 μl per trial and air-tight sealing for any type of protein sample. The ceiling crystals grow to sufficient size for diffraction experiments. In this highlight, we fully describe the features of this kit and how easily it can be used to yield protein crystals.